smartscribe reverse transcriptase kit  (TaKaRa)

Article Title: METTL3 mediates m 6 A methylation of ADAM17 to Downregulate ACE2–Ang-(1–7) axis and promote progression of hypertension
Article Snippet: A minimum of 1 μg total RNA was fragmented with an NEBNext Magnesium RNA fragmentation module (New England Biolabs, Ipswich, MA, USA) by incubating at 94 °C for 5 min. Fragmented RNA, including rRNA, was enriched with m6A antibody–Dynabead complexes through immunoprecipitation. .. Immunoprecipitated RNA was then reverse-transcribed to yield first-strand cDNA via SMARTScribe reverse transcriptase (ClonTech, Japan). ..

Article Title: METTL3 mediates m 6 A methylation of ADAM17 to Downregulate ACE2–Ang-(1–7) axis and promote progression of hypertension
Article Snippet: A minimum of 1 μg total RNA was fragmented with an NEBNext Magnesium RNA fragmentation module (New England Biolabs, Ipswich, MA, USA) by incubating at 94 °C for 5 min. Fragmented RNA, including rRNA, was enriched with m6A antibody–Dynabead complexes through immunoprecipitation. .. Immunoprecipitated RNA was then reverse-transcribed to yield first-strand cDNA via SMARTScribe reverse transcriptase (ClonTech, Japan). ..

Article Title: Pan-cancer macrophage atlas uncovers that MHC-II high TAMs induce Treg activation through physical interactions.
Article Snippet: For bulk RNA-seq, RNA extraction was performed using FastPure Cell/Tissue Total RNA Isolation Kit V2 (Vazyme RC112-01). .. Reverse transcription was performed using SMARTScribe Reverse Transcriptase (Clontech) according to the manufacturer’s instructions. .. The cDNA was amplified and the final library was constructed using TD501 kit (Vazyme).

Article Title: Mesenchymal stroma cell-derived stem cell factor mediates cross-species compatibility of the hematopoietic stem cell niche
Article Snippet: RNA was prepared using the miRNeasy Micro Kit (Qiagen). .. Total RNA was eluted in 10ul and 5ul were used for library preparation for next generation sequencing. cDNA was synthesized from 5 μl total RNA using the SmartScribe reverse transcriptase (Takara Bio, SMARTer HV Kit) with a universally tailed poly-dT primer and a template switching oligo followed by amplification for 12 cycles with the Advantage 2 DNA Polymerase (Takara Bio). .. After ultrasonic shearing (Covaris LE220), amplified cDNA samples were subjected to standard Illumina fragment library preparation using the NEBnext Ultra DNA library preparation chemistry (New England Biolabs).

Article Title: A Recombinant Antibody Against Human DRP1 Serine 616 Phosphorylation Enables Detection of BRAF V600E -Associated Mitochondrial Division in Cancer
Article Snippet: Sequencing of the 3G11 antibody was performed using SMARTer 5′ RACE technology (Takara Bio, MoutainView, CA, USA, Cat. No. 634858) adapted for immunoglobulins to amplify the variable genes from the heavy and kappa chains from the 3G11 hybridoma. .. Briefly, RNA was extracted from the hybridoma using a RNeasy Mini Kit (Qiagen, Hilden, Germany, Cat. No. 74104), followed by first-strand cDNA synthesis using constant gene-specific 3′ primers (GSP1) based on mouse IgG/mouse kappa constant isotypes and incubation with the SMARTer II A Oligonucleotide and SMARTscribe reverse transcriptase (Takara, MoutainView, CA, USA). ..

Article Title: Direct-to-library methods, systems, and compositions
Article Snippet: 2.0 μL 1 μM Poly dT primer (IDT) and 6 μL of SMARTScribe 1st Strand buffer (5×) (Takara, Kusatsu, Japan) was added to the eluate and the resulting mixture incubated at 95° C. for 1 minute before placing on ice. .. The Extension and Template-switching mix was prepared by combining 4.5 μL SMARTScribe 1st Strand buffer (5×) (Takara, Kusatsu, Japan), 0.5 μL dNTP mix (25 mM per nucleotide, Thermo-Fisher Scientific, Waltham, MA), 2.0 μL SMARTScribe Reverse Transcriptase (100 u/μL, Takara, Kusatsu, Japan), 2.0 μL 5 μM Template-switching Oligo (TS Oligo) (IDT), 5.0 μL of DTT (20M, Takara, Kusatsu, Japan), and 4 μL Nuclease-free water. .. The resulting reaction mixture was incubated for 90 min at 42° C. and the reaction was heat denatured at 70° C. for 15 min. Next, 50.0 μL of NEBNext Ultra II Q5 (NEB, Ipswich, MA), and 8.0 μL of indexing primer mixture (NEB, Ipswich, MA) were added to the reaction from the previous step.

Article Title: A standardized patient-derived glioblastoma organoid platform for evaluating radiotherapy efficacy and overcoming resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Doublecortin Santa Cruz Biotechnology Cat# sc-8066; RRID: AB_2088494 Goat anti-Olig2 R&D Systems Cat# AF2418; RRID: AB_2157554 Mouse anti-GFAP Sigma-Aldrich Cat# MAB360; RRID: AB_11212597 Mouse Anti-Ki-67 BD Biosciences Cat# 550609; RRID: AB_39377 Mouse anti-SOX9 Sigma-Aldrich Cat# AF146; RRID: AB_2239761 Rabbit anti-EGFRvIII Cell Signaling Cat# 64952 S; RRID: AB_2773018 Rabbit anti-HOPX Santa Cruz Biotechnology Cat# sc-30216; RRID: AB_2120833 Rat anti-Tenascin C R&D Systems Cat# MAB2138; RRID: AB_2203818 donkey anti-goat IgG Alexa Fluor 488 Thermo Fisher Cat# A-11055; RRID: AB_2336933 donkey anti-goat IgG Alexa Fluor 647 Thermo Fisher Cat# A-21447; RRID: AB_2535864 donkey anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Cat# A-31572; RRID: AB_162543 donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Cat# A-21202; RRID: AB_141607 donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher Cat# A-31570; RRID: AB_2536180 donkey anti-mouse IgG Alexa Fluor 647 Thermo Fisher Cat# A-31571; RRID: AB_162542 Chemicals, peptides, and recombinant proteins 1× RBC lysis buffer Thermo Fisher Scientific Cat# 00433357 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 ABBV-744 MedChemExpress Cat# HY-112090 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 AZD-5069 MedChemExpress Cat# HY-19855 B27 supplement without vitamin A Gibco Cat# 12587010 BGT226 MedChemExpress Cat# HY-13334 A BI-2536 MedChemExpress Cat# HY-50698 Bovine serum albumin (BSA) Sigma-Aldrich Cat# B6917; CAS# 9048–46–8 DAPI Sigma-Aldrich Cat# 10236276001; CAS# 28718–90–3 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650; CAS# 67–68–5 DMEM/F12 Medium Gibco Cat# 11330032 Donkey Serum Sigma-Aldrich Cat# S30-100ML Dulbecco’s phosphate-buffered saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat# 14190144 Formaldehyde, 16%, methanol free, Ultrapure Polysciences Cat# 18814–10; CAS#: 50–00–0 Foscarnet MedChemExpress Cat# HY-B1318 Geldanamycin MedChemExpress Cat# HY-15230 GlutaMAXTM Gibco Cat# 35050038 GSK778 MedChemExpress Cat# HY-136570 HibernateTM-A Medium Gibco Cat# A1247501 I-BET151 MedChemExpress Cat# HY-13235 Insulin solution human Sigma-Aldrich Cat# I9278 Ipatasertib MedChemExpress Cat# HY-15186 JQ-1 MedChemExpress Cat# HY-13030 KAPA SYBR FAST 2× MasterMix Thermo Fisher Scientific Cat# 50–196–5206 Masitinib MedChemExpress Cat# HY-10209 (Continued on next page) e1 Cell Reports Medicine 7, 103095, October 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Minimum Essential Medium from nonessential amino acids Gibco Cat# 11140050 N2 supplement Gibco Cat# 7502048 Neurobasal Medium Gibco Cat# 11570556 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 Paxalisib Selleckchem Cat# S8163 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 PFI-1 MedChemExpress Cat# HY-16586 RNA Clean & Concentrator 5 Zymo Research Cat# R1013 SB225002 MedChemExpress Cat# HY-16711 SMARTScribe Reverse Transcriptase Takara Cat# 639537 SPRIselect Reagent Beckman Coulter Cat# B23318 Tanespimycin MedChemExpress Cat# HY-10211 TritonTM X-100 Sigma-Aldrich Cat# T9284 Torin-2 MedChemExpress Cat# HY-13002 Tris (1 M), pH 8.0, RNase-free Thermo Fisher Scientific Cat# AM9855G TRIzol reagent Thermo Fisher Scientific Cat# 15596026 Trypan blue stain, 0.4% Thermo Fisher Scientific Cat# T10282 Tween 20 Sigma-Aldrich Cat# P9416-50mL; CAS# 9005–64–5 VECTASHIELD Vibrance Antifade Mounting Medium Vector Laboratories Cat# H170010 XMD8-92 Selleckchem Cat# S7525 Critical commercial assays Bioanalyzer high sensitivity DNA analysis Agilent Cat# 5067–4626 Brain Tumor Dissociation Kit Miltenyi Biotech Cat# 130–095–942 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9683 KAPA Library Quantification Kit for Illumina NGS Kapa Biosystems Cat# KK4835 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB Cat# E7490L NEBNext UltraTM II RNA Library Prep Kit for Illumina NEB Cat# E7775L Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q33231 Deposited data RNA sequencing of primary, GBO, and reaggregate GBOs This paper NCBI GEO Accession GEO: GSE300985 Experimental models: Cell lines Glioblastoma organoid samples This paper Table S1 Experimental models: Organisms/strains NU/J Mus musculus, female The Jackson Laboratory Cat# 002019; RRID: IMSR_JAX:002019 Software and algorithms Adobe Illustrator 28.2 64-bit Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_010279 bcl2fastq v.2.19.0.316 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html; RRID:SCR_015058 DESeq2 v.1.22.2 Love et al.41 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID:SCR_015687 (Continued on next page) Cell Reports Medicine 7, 103095, October 20, 2026 e2 Article ll OPEN ACCESS ..

Article Title: Direct-to-library methods, systems, and compositions
Article Snippet: .. For extension and template switching, 0.5×SMARTScribe RT buffer (Takara, Japan), 2 μM DTT (Takara, Japan), 0.25 mM dNTPs (Thermo-Fisher, Waltham, MA), 200 units of SMARTScribe Reverse Transcriptase (Takara Japan) and template switching oligo (0.2 uM, IDT DNA, Coralville, IA) was added and incubated for 90 minutes at 42° C. followed by 5 cycles of 50° C. for 2 minutes and 42° C. for 2 minutes and 5 cycles of 55° C. for 30 seconds and 42° C. for 2 minutes and then heat denatured at 70° C. for 15 minutes. .. This reaction was then amplified in 1× Terra Direct buffer (Takara, Japan) with 1.25 unit Terra Direct polymerase (Takara, Japan) and 200 nM PCR primers using the following conditions: 98° C. for 2 minutes, 12 cycles of 98° C. for 15 seconds, 60° C. for 30 seconds, 68° C. for 1 minute.

Article Title: The circadian isoform landscape of mouse livers
Article Snippet: .. First strand reverse transcription was performed for 1 h at 42°C, before being heat inactivated for 5 min at 70°C, using the Smartscribe Reverse Transcriptase (Clontech). ..

Article Title: Mesenchymal stroma cell-derived stem cell factor mediates cross-species compatibility of the hematopoietic stem cell niche
Article Snippet: RNA was prepared using the miRNeasy Micro Kit (Qiagen). .. Total RNA was eluted in 10ul and 5ul were used for library preparation for next generation sequencing. cDNA was synthesized from 5 μl total RNA using the SmartScribe reverse transcriptase (Takara Bio, SMARTer HV Kit) with a universally tailed poly-dT primer and a template switching oligo followed by amplification for 12 cycles with the Advantage 2 DNA Polymerase (Takara Bio). .. After ultrasonic shearing (Covaris LE220), amplified cDNA samples were subjected to standard Illumina fragment library preparation using the NEBnext Ultra DNA library preparation chemistry (New England Biolabs).

Article Title: A standardized patient-derived glioblastoma organoid platform for evaluating radiotherapy efficacy and overcoming resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Doublecortin Santa Cruz Biotechnology Cat# sc-8066; RRID: AB_2088494 Goat anti-Olig2 R&D Systems Cat# AF2418; RRID: AB_2157554 Mouse anti-GFAP Sigma-Aldrich Cat# MAB360; RRID: AB_11212597 Mouse Anti-Ki-67 BD Biosciences Cat# 550609; RRID: AB_39377 Mouse anti-SOX9 Sigma-Aldrich Cat# AF146; RRID: AB_2239761 Rabbit anti-EGFRvIII Cell Signaling Cat# 64952 S; RRID: AB_2773018 Rabbit anti-HOPX Santa Cruz Biotechnology Cat# sc-30216; RRID: AB_2120833 Rat anti-Tenascin C R&D Systems Cat# MAB2138; RRID: AB_2203818 donkey anti-goat IgG Alexa Fluor 488 Thermo Fisher Cat# A-11055; RRID: AB_2336933 donkey anti-goat IgG Alexa Fluor 647 Thermo Fisher Cat# A-21447; RRID: AB_2535864 donkey anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Cat# A-31572; RRID: AB_162543 donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Cat# A-21202; RRID: AB_141607 donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher Cat# A-31570; RRID: AB_2536180 donkey anti-mouse IgG Alexa Fluor 647 Thermo Fisher Cat# A-31571; RRID: AB_162542 Chemicals, peptides, and recombinant proteins 1× RBC lysis buffer Thermo Fisher Scientific Cat# 00433357 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 ABBV-744 MedChemExpress Cat# HY-112090 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 AZD-5069 MedChemExpress Cat# HY-19855 B27 supplement without vitamin A Gibco Cat# 12587010 BGT226 MedChemExpress Cat# HY-13334 A BI-2536 MedChemExpress Cat# HY-50698 Bovine serum albumin (BSA) Sigma-Aldrich Cat# B6917; CAS# 9048–46–8 DAPI Sigma-Aldrich Cat# 10236276001; CAS# 28718–90–3 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650; CAS# 67–68–5 DMEM/F12 Medium Gibco Cat# 11330032 Donkey Serum Sigma-Aldrich Cat# S30-100ML Dulbecco’s phosphate-buffered saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat# 14190144 Formaldehyde, 16%, methanol free, Ultrapure Polysciences Cat# 18814–10; CAS#: 50–00–0 Foscarnet MedChemExpress Cat# HY-B1318 Geldanamycin MedChemExpress Cat# HY-15230 GlutaMAXTM Gibco Cat# 35050038 GSK778 MedChemExpress Cat# HY-136570 HibernateTM-A Medium Gibco Cat# A1247501 I-BET151 MedChemExpress Cat# HY-13235 Insulin solution human Sigma-Aldrich Cat# I9278 Ipatasertib MedChemExpress Cat# HY-15186 JQ-1 MedChemExpress Cat# HY-13030 KAPA SYBR FAST 2× MasterMix Thermo Fisher Scientific Cat# 50–196–5206 Masitinib MedChemExpress Cat# HY-10209 (Continued on next page) e1 Cell Reports Medicine 7, 103095, October 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Minimum Essential Medium from nonessential amino acids Gibco Cat# 11140050 N2 supplement Gibco Cat# 7502048 Neurobasal Medium Gibco Cat# 11570556 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 Paxalisib Selleckchem Cat# S8163 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 PFI-1 MedChemExpress Cat# HY-16586 RNA Clean & Concentrator 5 Zymo Research Cat# R1013 SB225002 MedChemExpress Cat# HY-16711 SMARTScribe Reverse Transcriptase Takara Cat# 639537 SPRIselect Reagent Beckman Coulter Cat# B23318 Tanespimycin MedChemExpress Cat# HY-10211 TritonTM X-100 Sigma-Aldrich Cat# T9284 Torin-2 MedChemExpress Cat# HY-13002 Tris (1 M), pH 8.0, RNase-free Thermo Fisher Scientific Cat# AM9855G TRIzol reagent Thermo Fisher Scientific Cat# 15596026 Trypan blue stain, 0.4% Thermo Fisher Scientific Cat# T10282 Tween 20 Sigma-Aldrich Cat# P9416-50mL; CAS# 9005–64–5 VECTASHIELD Vibrance Antifade Mounting Medium Vector Laboratories Cat# H170010 XMD8-92 Selleckchem Cat# S7525 Critical commercial assays Bioanalyzer high sensitivity DNA analysis Agilent Cat# 5067–4626 Brain Tumor Dissociation Kit Miltenyi Biotech Cat# 130–095–942 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9683 KAPA Library Quantification Kit for Illumina NGS Kapa Biosystems Cat# KK4835 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB Cat# E7490L NEBNext UltraTM II RNA Library Prep Kit for Illumina NEB Cat# E7775L Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q33231 Deposited data RNA sequencing of primary, GBO, and reaggregate GBOs This paper NCBI GEO Accession GEO: GSE300985 Experimental models: Cell lines Glioblastoma organoid samples This paper Table S1 Experimental models: Organisms/strains NU/J Mus musculus, female The Jackson Laboratory Cat# 002019; RRID: IMSR_JAX:002019 Software and algorithms Adobe Illustrator 28.2 64-bit Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_010279 bcl2fastq v.2.19.0.316 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html; RRID:SCR_015058 DESeq2 v.1.22.2 Love et al.41 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID:SCR_015687 (Continued on next page) Cell Reports Medicine 7, 103095, October 20, 2026 e2 Article ll OPEN ACCESS ..

Article Title: Mesenchymal stroma cell-derived stem cell factor mediates cross-species compatibility of the hematopoietic stem cell niche
Article Snippet: RNA was prepared using the miRNeasy Micro Kit (Qiagen). .. Total RNA was eluted in 10ul and 5ul were used for library preparation for next generation sequencing. cDNA was synthesized from 5 μl total RNA using the SmartScribe reverse transcriptase (Takara Bio, SMARTer HV Kit) with a universally tailed poly-dT primer and a template switching oligo followed by amplification for 12 cycles with the Advantage 2 DNA Polymerase (Takara Bio). .. After ultrasonic shearing (Covaris LE220), amplified cDNA samples were subjected to standard Illumina fragment library preparation using the NEBnext Ultra DNA library preparation chemistry (New England Biolabs).

Article Title: Mesenchymal stroma cell-derived stem cell factor mediates cross-species compatibility of the hematopoietic stem cell niche
Article Snippet: RNA was prepared using the miRNeasy Micro Kit (Qiagen). .. Total RNA was eluted in 10ul and 5ul were used for library preparation for next generation sequencing. cDNA was synthesized from 5 μl total RNA using the SmartScribe reverse transcriptase (Takara Bio, SMARTer HV Kit) with a universally tailed poly-dT primer and a template switching oligo followed by amplification for 12 cycles with the Advantage 2 DNA Polymerase (Takara Bio). .. After ultrasonic shearing (Covaris LE220), amplified cDNA samples were subjected to standard Illumina fragment library preparation using the NEBnext Ultra DNA library preparation chemistry (New England Biolabs).

Article Title: A Recombinant Antibody Against Human DRP1 Serine 616 Phosphorylation Enables Detection of BRAF V600E -Associated Mitochondrial Division in Cancer
Article Snippet: Sequencing of the 3G11 antibody was performed using SMARTer 5′ RACE technology (Takara Bio, MoutainView, CA, USA, Cat. No. 634858) adapted for immunoglobulins to amplify the variable genes from the heavy and kappa chains from the 3G11 hybridoma. .. Briefly, RNA was extracted from the hybridoma using a RNeasy Mini Kit (Qiagen, Hilden, Germany, Cat. No. 74104), followed by first-strand cDNA synthesis using constant gene-specific 3′ primers (GSP1) based on mouse IgG/mouse kappa constant isotypes and incubation with the SMARTer II A Oligonucleotide and SMARTscribe reverse transcriptase (Takara, MoutainView, CA, USA). ..

Article Title: A Recombinant Antibody Against Human DRP1 Serine 616 Phosphorylation Enables Detection of BRAF V600E -Associated Mitochondrial Division in Cancer
Article Snippet: Sequencing of the 3G11 antibody was performed using SMARTer 5′ RACE technology (Takara Bio, MoutainView, CA, USA, Cat. No. 634858) adapted for immunoglobulins to amplify the variable genes from the heavy and kappa chains from the 3G11 hybridoma. .. Briefly, RNA was extracted from the hybridoma using a RNeasy Mini Kit (Qiagen, Hilden, Germany, Cat. No. 74104), followed by first-strand cDNA synthesis using constant gene-specific 3′ primers (GSP1) based on mouse IgG/mouse kappa constant isotypes and incubation with the SMARTer II A Oligonucleotide and SMARTscribe reverse transcriptase (Takara, MoutainView, CA, USA). ..

Article Title: Direct-to-library methods, systems, and compositions
Article Snippet: .. For extension and template switching, 0.5×SMARTScribe RT buffer (Takara, Japan), 2 μM DTT (Takara, Japan), 0.25 mM dNTPs (Thermo-Fisher, Waltham, MA), 200 units of SMARTScribe Reverse Transcriptase (Takara Japan) and template switching oligo (0.2 uM, IDT DNA, Coralville, IA) was added and incubated for 90 minutes at 42° C. followed by 5 cycles of 50° C. for 2 minutes and 42° C. for 2 minutes and 5 cycles of 55° C. for 30 seconds and 42° C. for 2 minutes and then heat denatured at 70° C. for 15 minutes. .. This reaction was then amplified in 1× Terra Direct buffer (Takara, Japan) with 1.25 unit Terra Direct polymerase (Takara, Japan) and 200 nM PCR primers using the following conditions: 98° C. for 2 minutes, 12 cycles of 98° C. for 15 seconds, 60° C. for 30 seconds, 68° C. for 1 minute.

Article Title: A standardized patient-derived glioblastoma organoid platform for evaluating radiotherapy efficacy and overcoming resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Doublecortin Santa Cruz Biotechnology Cat# sc-8066; RRID: AB_2088494 Goat anti-Olig2 R&D Systems Cat# AF2418; RRID: AB_2157554 Mouse anti-GFAP Sigma-Aldrich Cat# MAB360; RRID: AB_11212597 Mouse Anti-Ki-67 BD Biosciences Cat# 550609; RRID: AB_39377 Mouse anti-SOX9 Sigma-Aldrich Cat# AF146; RRID: AB_2239761 Rabbit anti-EGFRvIII Cell Signaling Cat# 64952 S; RRID: AB_2773018 Rabbit anti-HOPX Santa Cruz Biotechnology Cat# sc-30216; RRID: AB_2120833 Rat anti-Tenascin C R&D Systems Cat# MAB2138; RRID: AB_2203818 donkey anti-goat IgG Alexa Fluor 488 Thermo Fisher Cat# A-11055; RRID: AB_2336933 donkey anti-goat IgG Alexa Fluor 647 Thermo Fisher Cat# A-21447; RRID: AB_2535864 donkey anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Cat# A-31572; RRID: AB_162543 donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Cat# A-21202; RRID: AB_141607 donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher Cat# A-31570; RRID: AB_2536180 donkey anti-mouse IgG Alexa Fluor 647 Thermo Fisher Cat# A-31571; RRID: AB_162542 Chemicals, peptides, and recombinant proteins 1× RBC lysis buffer Thermo Fisher Scientific Cat# 00433357 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 ABBV-744 MedChemExpress Cat# HY-112090 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 AZD-5069 MedChemExpress Cat# HY-19855 B27 supplement without vitamin A Gibco Cat# 12587010 BGT226 MedChemExpress Cat# HY-13334 A BI-2536 MedChemExpress Cat# HY-50698 Bovine serum albumin (BSA) Sigma-Aldrich Cat# B6917; CAS# 9048–46–8 DAPI Sigma-Aldrich Cat# 10236276001; CAS# 28718–90–3 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650; CAS# 67–68–5 DMEM/F12 Medium Gibco Cat# 11330032 Donkey Serum Sigma-Aldrich Cat# S30-100ML Dulbecco’s phosphate-buffered saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat# 14190144 Formaldehyde, 16%, methanol free, Ultrapure Polysciences Cat# 18814–10; CAS#: 50–00–0 Foscarnet MedChemExpress Cat# HY-B1318 Geldanamycin MedChemExpress Cat# HY-15230 GlutaMAXTM Gibco Cat# 35050038 GSK778 MedChemExpress Cat# HY-136570 HibernateTM-A Medium Gibco Cat# A1247501 I-BET151 MedChemExpress Cat# HY-13235 Insulin solution human Sigma-Aldrich Cat# I9278 Ipatasertib MedChemExpress Cat# HY-15186 JQ-1 MedChemExpress Cat# HY-13030 KAPA SYBR FAST 2× MasterMix Thermo Fisher Scientific Cat# 50–196–5206 Masitinib MedChemExpress Cat# HY-10209 (Continued on next page) e1 Cell Reports Medicine 7, 103095, October 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Minimum Essential Medium from nonessential amino acids Gibco Cat# 11140050 N2 supplement Gibco Cat# 7502048 Neurobasal Medium Gibco Cat# 11570556 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 Paxalisib Selleckchem Cat# S8163 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 PFI-1 MedChemExpress Cat# HY-16586 RNA Clean & Concentrator 5 Zymo Research Cat# R1013 SB225002 MedChemExpress Cat# HY-16711 SMARTScribe Reverse Transcriptase Takara Cat# 639537 SPRIselect Reagent Beckman Coulter Cat# B23318 Tanespimycin MedChemExpress Cat# HY-10211 TritonTM X-100 Sigma-Aldrich Cat# T9284 Torin-2 MedChemExpress Cat# HY-13002 Tris (1 M), pH 8.0, RNase-free Thermo Fisher Scientific Cat# AM9855G TRIzol reagent Thermo Fisher Scientific Cat# 15596026 Trypan blue stain, 0.4% Thermo Fisher Scientific Cat# T10282 Tween 20 Sigma-Aldrich Cat# P9416-50mL; CAS# 9005–64–5 VECTASHIELD Vibrance Antifade Mounting Medium Vector Laboratories Cat# H170010 XMD8-92 Selleckchem Cat# S7525 Critical commercial assays Bioanalyzer high sensitivity DNA analysis Agilent Cat# 5067–4626 Brain Tumor Dissociation Kit Miltenyi Biotech Cat# 130–095–942 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9683 KAPA Library Quantification Kit for Illumina NGS Kapa Biosystems Cat# KK4835 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB Cat# E7490L NEBNext UltraTM II RNA Library Prep Kit for Illumina NEB Cat# E7775L Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q33231 Deposited data RNA sequencing of primary, GBO, and reaggregate GBOs This paper NCBI GEO Accession GEO: GSE300985 Experimental models: Cell lines Glioblastoma organoid samples This paper Table S1 Experimental models: Organisms/strains NU/J Mus musculus, female The Jackson Laboratory Cat# 002019; RRID: IMSR_JAX:002019 Software and algorithms Adobe Illustrator 28.2 64-bit Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_010279 bcl2fastq v.2.19.0.316 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html; RRID:SCR_015058 DESeq2 v.1.22.2 Love et al.41 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID:SCR_015687 (Continued on next page) Cell Reports Medicine 7, 103095, October 20, 2026 e2 Article ll OPEN ACCESS ..

Article Title: A standardized patient-derived glioblastoma organoid platform for evaluating radiotherapy efficacy and overcoming resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Doublecortin Santa Cruz Biotechnology Cat# sc-8066; RRID: AB_2088494 Goat anti-Olig2 R&D Systems Cat# AF2418; RRID: AB_2157554 Mouse anti-GFAP Sigma-Aldrich Cat# MAB360; RRID: AB_11212597 Mouse Anti-Ki-67 BD Biosciences Cat# 550609; RRID: AB_39377 Mouse anti-SOX9 Sigma-Aldrich Cat# AF146; RRID: AB_2239761 Rabbit anti-EGFRvIII Cell Signaling Cat# 64952 S; RRID: AB_2773018 Rabbit anti-HOPX Santa Cruz Biotechnology Cat# sc-30216; RRID: AB_2120833 Rat anti-Tenascin C R&D Systems Cat# MAB2138; RRID: AB_2203818 donkey anti-goat IgG Alexa Fluor 488 Thermo Fisher Cat# A-11055; RRID: AB_2336933 donkey anti-goat IgG Alexa Fluor 647 Thermo Fisher Cat# A-21447; RRID: AB_2535864 donkey anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Cat# A-31572; RRID: AB_162543 donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Cat# A-21202; RRID: AB_141607 donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher Cat# A-31570; RRID: AB_2536180 donkey anti-mouse IgG Alexa Fluor 647 Thermo Fisher Cat# A-31571; RRID: AB_162542 Chemicals, peptides, and recombinant proteins 1× RBC lysis buffer Thermo Fisher Scientific Cat# 00433357 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 ABBV-744 MedChemExpress Cat# HY-112090 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 AZD-5069 MedChemExpress Cat# HY-19855 B27 supplement without vitamin A Gibco Cat# 12587010 BGT226 MedChemExpress Cat# HY-13334 A BI-2536 MedChemExpress Cat# HY-50698 Bovine serum albumin (BSA) Sigma-Aldrich Cat# B6917; CAS# 9048–46–8 DAPI Sigma-Aldrich Cat# 10236276001; CAS# 28718–90–3 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650; CAS# 67–68–5 DMEM/F12 Medium Gibco Cat# 11330032 Donkey Serum Sigma-Aldrich Cat# S30-100ML Dulbecco’s phosphate-buffered saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat# 14190144 Formaldehyde, 16%, methanol free, Ultrapure Polysciences Cat# 18814–10; CAS#: 50–00–0 Foscarnet MedChemExpress Cat# HY-B1318 Geldanamycin MedChemExpress Cat# HY-15230 GlutaMAXTM Gibco Cat# 35050038 GSK778 MedChemExpress Cat# HY-136570 HibernateTM-A Medium Gibco Cat# A1247501 I-BET151 MedChemExpress Cat# HY-13235 Insulin solution human Sigma-Aldrich Cat# I9278 Ipatasertib MedChemExpress Cat# HY-15186 JQ-1 MedChemExpress Cat# HY-13030 KAPA SYBR FAST 2× MasterMix Thermo Fisher Scientific Cat# 50–196–5206 Masitinib MedChemExpress Cat# HY-10209 (Continued on next page) e1 Cell Reports Medicine 7, 103095, October 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Minimum Essential Medium from nonessential amino acids Gibco Cat# 11140050 N2 supplement Gibco Cat# 7502048 Neurobasal Medium Gibco Cat# 11570556 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 Paxalisib Selleckchem Cat# S8163 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 PFI-1 MedChemExpress Cat# HY-16586 RNA Clean & Concentrator 5 Zymo Research Cat# R1013 SB225002 MedChemExpress Cat# HY-16711 SMARTScribe Reverse Transcriptase Takara Cat# 639537 SPRIselect Reagent Beckman Coulter Cat# B23318 Tanespimycin MedChemExpress Cat# HY-10211 TritonTM X-100 Sigma-Aldrich Cat# T9284 Torin-2 MedChemExpress Cat# HY-13002 Tris (1 M), pH 8.0, RNase-free Thermo Fisher Scientific Cat# AM9855G TRIzol reagent Thermo Fisher Scientific Cat# 15596026 Trypan blue stain, 0.4% Thermo Fisher Scientific Cat# T10282 Tween 20 Sigma-Aldrich Cat# P9416-50mL; CAS# 9005–64–5 VECTASHIELD Vibrance Antifade Mounting Medium Vector Laboratories Cat# H170010 XMD8-92 Selleckchem Cat# S7525 Critical commercial assays Bioanalyzer high sensitivity DNA analysis Agilent Cat# 5067–4626 Brain Tumor Dissociation Kit Miltenyi Biotech Cat# 130–095–942 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9683 KAPA Library Quantification Kit for Illumina NGS Kapa Biosystems Cat# KK4835 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB Cat# E7490L NEBNext UltraTM II RNA Library Prep Kit for Illumina NEB Cat# E7775L Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q33231 Deposited data RNA sequencing of primary, GBO, and reaggregate GBOs This paper NCBI GEO Accession GEO: GSE300985 Experimental models: Cell lines Glioblastoma organoid samples This paper Table S1 Experimental models: Organisms/strains NU/J Mus musculus, female The Jackson Laboratory Cat# 002019; RRID: IMSR_JAX:002019 Software and algorithms Adobe Illustrator 28.2 64-bit Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_010279 bcl2fastq v.2.19.0.316 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html; RRID:SCR_015058 DESeq2 v.1.22.2 Love et al.41 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID:SCR_015687 (Continued on next page) Cell Reports Medicine 7, 103095, October 20, 2026 e2 Article ll OPEN ACCESS ..

Article Title: A standardized patient-derived glioblastoma organoid platform for evaluating radiotherapy efficacy and overcoming resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Doublecortin Santa Cruz Biotechnology Cat# sc-8066; RRID: AB_2088494 Goat anti-Olig2 R&D Systems Cat# AF2418; RRID: AB_2157554 Mouse anti-GFAP Sigma-Aldrich Cat# MAB360; RRID: AB_11212597 Mouse Anti-Ki-67 BD Biosciences Cat# 550609; RRID: AB_39377 Mouse anti-SOX9 Sigma-Aldrich Cat# AF146; RRID: AB_2239761 Rabbit anti-EGFRvIII Cell Signaling Cat# 64952 S; RRID: AB_2773018 Rabbit anti-HOPX Santa Cruz Biotechnology Cat# sc-30216; RRID: AB_2120833 Rat anti-Tenascin C R&D Systems Cat# MAB2138; RRID: AB_2203818 donkey anti-goat IgG Alexa Fluor 488 Thermo Fisher Cat# A-11055; RRID: AB_2336933 donkey anti-goat IgG Alexa Fluor 647 Thermo Fisher Cat# A-21447; RRID: AB_2535864 donkey anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Cat# A-31572; RRID: AB_162543 donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Cat# A-21202; RRID: AB_141607 donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher Cat# A-31570; RRID: AB_2536180 donkey anti-mouse IgG Alexa Fluor 647 Thermo Fisher Cat# A-31571; RRID: AB_162542 Chemicals, peptides, and recombinant proteins 1× RBC lysis buffer Thermo Fisher Scientific Cat# 00433357 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 ABBV-744 MedChemExpress Cat# HY-112090 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 AZD-5069 MedChemExpress Cat# HY-19855 B27 supplement without vitamin A Gibco Cat# 12587010 BGT226 MedChemExpress Cat# HY-13334 A BI-2536 MedChemExpress Cat# HY-50698 Bovine serum albumin (BSA) Sigma-Aldrich Cat# B6917; CAS# 9048–46–8 DAPI Sigma-Aldrich Cat# 10236276001; CAS# 28718–90–3 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650; CAS# 67–68–5 DMEM/F12 Medium Gibco Cat# 11330032 Donkey Serum Sigma-Aldrich Cat# S30-100ML Dulbecco’s phosphate-buffered saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat# 14190144 Formaldehyde, 16%, methanol free, Ultrapure Polysciences Cat# 18814–10; CAS#: 50–00–0 Foscarnet MedChemExpress Cat# HY-B1318 Geldanamycin MedChemExpress Cat# HY-15230 GlutaMAXTM Gibco Cat# 35050038 GSK778 MedChemExpress Cat# HY-136570 HibernateTM-A Medium Gibco Cat# A1247501 I-BET151 MedChemExpress Cat# HY-13235 Insulin solution human Sigma-Aldrich Cat# I9278 Ipatasertib MedChemExpress Cat# HY-15186 JQ-1 MedChemExpress Cat# HY-13030 KAPA SYBR FAST 2× MasterMix Thermo Fisher Scientific Cat# 50–196–5206 Masitinib MedChemExpress Cat# HY-10209 (Continued on next page) e1 Cell Reports Medicine 7, 103095, October 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Minimum Essential Medium from nonessential amino acids Gibco Cat# 11140050 N2 supplement Gibco Cat# 7502048 Neurobasal Medium Gibco Cat# 11570556 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 Paxalisib Selleckchem Cat# S8163 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 PFI-1 MedChemExpress Cat# HY-16586 RNA Clean & Concentrator 5 Zymo Research Cat# R1013 SB225002 MedChemExpress Cat# HY-16711 SMARTScribe Reverse Transcriptase Takara Cat# 639537 SPRIselect Reagent Beckman Coulter Cat# B23318 Tanespimycin MedChemExpress Cat# HY-10211 TritonTM X-100 Sigma-Aldrich Cat# T9284 Torin-2 MedChemExpress Cat# HY-13002 Tris (1 M), pH 8.0, RNase-free Thermo Fisher Scientific Cat# AM9855G TRIzol reagent Thermo Fisher Scientific Cat# 15596026 Trypan blue stain, 0.4% Thermo Fisher Scientific Cat# T10282 Tween 20 Sigma-Aldrich Cat# P9416-50mL; CAS# 9005–64–5 VECTASHIELD Vibrance Antifade Mounting Medium Vector Laboratories Cat# H170010 XMD8-92 Selleckchem Cat# S7525 Critical commercial assays Bioanalyzer high sensitivity DNA analysis Agilent Cat# 5067–4626 Brain Tumor Dissociation Kit Miltenyi Biotech Cat# 130–095–942 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9683 KAPA Library Quantification Kit for Illumina NGS Kapa Biosystems Cat# KK4835 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB Cat# E7490L NEBNext UltraTM II RNA Library Prep Kit for Illumina NEB Cat# E7775L Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q33231 Deposited data RNA sequencing of primary, GBO, and reaggregate GBOs This paper NCBI GEO Accession GEO: GSE300985 Experimental models: Cell lines Glioblastoma organoid samples This paper Table S1 Experimental models: Organisms/strains NU/J Mus musculus, female The Jackson Laboratory Cat# 002019; RRID: IMSR_JAX:002019 Software and algorithms Adobe Illustrator 28.2 64-bit Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_010279 bcl2fastq v.2.19.0.316 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html; RRID:SCR_015058 DESeq2 v.1.22.2 Love et al.41 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID:SCR_015687 (Continued on next page) Cell Reports Medicine 7, 103095, October 20, 2026 e2 Article ll OPEN ACCESS ..

Article Title: A standardized patient-derived glioblastoma organoid platform for evaluating radiotherapy efficacy and overcoming resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Doublecortin Santa Cruz Biotechnology Cat# sc-8066; RRID: AB_2088494 Goat anti-Olig2 R&D Systems Cat# AF2418; RRID: AB_2157554 Mouse anti-GFAP Sigma-Aldrich Cat# MAB360; RRID: AB_11212597 Mouse Anti-Ki-67 BD Biosciences Cat# 550609; RRID: AB_39377 Mouse anti-SOX9 Sigma-Aldrich Cat# AF146; RRID: AB_2239761 Rabbit anti-EGFRvIII Cell Signaling Cat# 64952 S; RRID: AB_2773018 Rabbit anti-HOPX Santa Cruz Biotechnology Cat# sc-30216; RRID: AB_2120833 Rat anti-Tenascin C R&D Systems Cat# MAB2138; RRID: AB_2203818 donkey anti-goat IgG Alexa Fluor 488 Thermo Fisher Cat# A-11055; RRID: AB_2336933 donkey anti-goat IgG Alexa Fluor 647 Thermo Fisher Cat# A-21447; RRID: AB_2535864 donkey anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Cat# A-31572; RRID: AB_162543 donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Cat# A-21202; RRID: AB_141607 donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher Cat# A-31570; RRID: AB_2536180 donkey anti-mouse IgG Alexa Fluor 647 Thermo Fisher Cat# A-31571; RRID: AB_162542 Chemicals, peptides, and recombinant proteins 1× RBC lysis buffer Thermo Fisher Scientific Cat# 00433357 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 ABBV-744 MedChemExpress Cat# HY-112090 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 AZD-5069 MedChemExpress Cat# HY-19855 B27 supplement without vitamin A Gibco Cat# 12587010 BGT226 MedChemExpress Cat# HY-13334 A BI-2536 MedChemExpress Cat# HY-50698 Bovine serum albumin (BSA) Sigma-Aldrich Cat# B6917; CAS# 9048–46–8 DAPI Sigma-Aldrich Cat# 10236276001; CAS# 28718–90–3 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650; CAS# 67–68–5 DMEM/F12 Medium Gibco Cat# 11330032 Donkey Serum Sigma-Aldrich Cat# S30-100ML Dulbecco’s phosphate-buffered saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat# 14190144 Formaldehyde, 16%, methanol free, Ultrapure Polysciences Cat# 18814–10; CAS#: 50–00–0 Foscarnet MedChemExpress Cat# HY-B1318 Geldanamycin MedChemExpress Cat# HY-15230 GlutaMAXTM Gibco Cat# 35050038 GSK778 MedChemExpress Cat# HY-136570 HibernateTM-A Medium Gibco Cat# A1247501 I-BET151 MedChemExpress Cat# HY-13235 Insulin solution human Sigma-Aldrich Cat# I9278 Ipatasertib MedChemExpress Cat# HY-15186 JQ-1 MedChemExpress Cat# HY-13030 KAPA SYBR FAST 2× MasterMix Thermo Fisher Scientific Cat# 50–196–5206 Masitinib MedChemExpress Cat# HY-10209 (Continued on next page) e1 Cell Reports Medicine 7, 103095, October 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Minimum Essential Medium from nonessential amino acids Gibco Cat# 11140050 N2 supplement Gibco Cat# 7502048 Neurobasal Medium Gibco Cat# 11570556 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 Paxalisib Selleckchem Cat# S8163 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 PFI-1 MedChemExpress Cat# HY-16586 RNA Clean & Concentrator 5 Zymo Research Cat# R1013 SB225002 MedChemExpress Cat# HY-16711 SMARTScribe Reverse Transcriptase Takara Cat# 639537 SPRIselect Reagent Beckman Coulter Cat# B23318 Tanespimycin MedChemExpress Cat# HY-10211 TritonTM X-100 Sigma-Aldrich Cat# T9284 Torin-2 MedChemExpress Cat# HY-13002 Tris (1 M), pH 8.0, RNase-free Thermo Fisher Scientific Cat# AM9855G TRIzol reagent Thermo Fisher Scientific Cat# 15596026 Trypan blue stain, 0.4% Thermo Fisher Scientific Cat# T10282 Tween 20 Sigma-Aldrich Cat# P9416-50mL; CAS# 9005–64–5 VECTASHIELD Vibrance Antifade Mounting Medium Vector Laboratories Cat# H170010 XMD8-92 Selleckchem Cat# S7525 Critical commercial assays Bioanalyzer high sensitivity DNA analysis Agilent Cat# 5067–4626 Brain Tumor Dissociation Kit Miltenyi Biotech Cat# 130–095–942 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9683 KAPA Library Quantification Kit for Illumina NGS Kapa Biosystems Cat# KK4835 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB Cat# E7490L NEBNext UltraTM II RNA Library Prep Kit for Illumina NEB Cat# E7775L Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q33231 Deposited data RNA sequencing of primary, GBO, and reaggregate GBOs This paper NCBI GEO Accession GEO: GSE300985 Experimental models: Cell lines Glioblastoma organoid samples This paper Table S1 Experimental models: Organisms/strains NU/J Mus musculus, female The Jackson Laboratory Cat# 002019; RRID: IMSR_JAX:002019 Software and algorithms Adobe Illustrator 28.2 64-bit Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_010279 bcl2fastq v.2.19.0.316 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html; RRID:SCR_015058 DESeq2 v.1.22.2 Love et al.41 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID:SCR_015687 (Continued on next page) Cell Reports Medicine 7, 103095, October 20, 2026 e2 Article ll OPEN ACCESS ..

Article Title: A standardized patient-derived glioblastoma organoid platform for evaluating radiotherapy efficacy and overcoming resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Doublecortin Santa Cruz Biotechnology Cat# sc-8066; RRID: AB_2088494 Goat anti-Olig2 R&D Systems Cat# AF2418; RRID: AB_2157554 Mouse anti-GFAP Sigma-Aldrich Cat# MAB360; RRID: AB_11212597 Mouse Anti-Ki-67 BD Biosciences Cat# 550609; RRID: AB_39377 Mouse anti-SOX9 Sigma-Aldrich Cat# AF146; RRID: AB_2239761 Rabbit anti-EGFRvIII Cell Signaling Cat# 64952 S; RRID: AB_2773018 Rabbit anti-HOPX Santa Cruz Biotechnology Cat# sc-30216; RRID: AB_2120833 Rat anti-Tenascin C R&D Systems Cat# MAB2138; RRID: AB_2203818 donkey anti-goat IgG Alexa Fluor 488 Thermo Fisher Cat# A-11055; RRID: AB_2336933 donkey anti-goat IgG Alexa Fluor 647 Thermo Fisher Cat# A-21447; RRID: AB_2535864 donkey anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Cat# A-31572; RRID: AB_162543 donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Cat# A-21202; RRID: AB_141607 donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher Cat# A-31570; RRID: AB_2536180 donkey anti-mouse IgG Alexa Fluor 647 Thermo Fisher Cat# A-31571; RRID: AB_162542 Chemicals, peptides, and recombinant proteins 1× RBC lysis buffer Thermo Fisher Scientific Cat# 00433357 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 ABBV-744 MedChemExpress Cat# HY-112090 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 AZD-5069 MedChemExpress Cat# HY-19855 B27 supplement without vitamin A Gibco Cat# 12587010 BGT226 MedChemExpress Cat# HY-13334 A BI-2536 MedChemExpress Cat# HY-50698 Bovine serum albumin (BSA) Sigma-Aldrich Cat# B6917; CAS# 9048–46–8 DAPI Sigma-Aldrich Cat# 10236276001; CAS# 28718–90–3 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650; CAS# 67–68–5 DMEM/F12 Medium Gibco Cat# 11330032 Donkey Serum Sigma-Aldrich Cat# S30-100ML Dulbecco’s phosphate-buffered saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat# 14190144 Formaldehyde, 16%, methanol free, Ultrapure Polysciences Cat# 18814–10; CAS#: 50–00–0 Foscarnet MedChemExpress Cat# HY-B1318 Geldanamycin MedChemExpress Cat# HY-15230 GlutaMAXTM Gibco Cat# 35050038 GSK778 MedChemExpress Cat# HY-136570 HibernateTM-A Medium Gibco Cat# A1247501 I-BET151 MedChemExpress Cat# HY-13235 Insulin solution human Sigma-Aldrich Cat# I9278 Ipatasertib MedChemExpress Cat# HY-15186 JQ-1 MedChemExpress Cat# HY-13030 KAPA SYBR FAST 2× MasterMix Thermo Fisher Scientific Cat# 50–196–5206 Masitinib MedChemExpress Cat# HY-10209 (Continued on next page) e1 Cell Reports Medicine 7, 103095, October 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Minimum Essential Medium from nonessential amino acids Gibco Cat# 11140050 N2 supplement Gibco Cat# 7502048 Neurobasal Medium Gibco Cat# 11570556 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 Paxalisib Selleckchem Cat# S8163 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 PFI-1 MedChemExpress Cat# HY-16586 RNA Clean & Concentrator 5 Zymo Research Cat# R1013 SB225002 MedChemExpress Cat# HY-16711 SMARTScribe Reverse Transcriptase Takara Cat# 639537 SPRIselect Reagent Beckman Coulter Cat# B23318 Tanespimycin MedChemExpress Cat# HY-10211 TritonTM X-100 Sigma-Aldrich Cat# T9284 Torin-2 MedChemExpress Cat# HY-13002 Tris (1 M), pH 8.0, RNase-free Thermo Fisher Scientific Cat# AM9855G TRIzol reagent Thermo Fisher Scientific Cat# 15596026 Trypan blue stain, 0.4% Thermo Fisher Scientific Cat# T10282 Tween 20 Sigma-Aldrich Cat# P9416-50mL; CAS# 9005–64–5 VECTASHIELD Vibrance Antifade Mounting Medium Vector Laboratories Cat# H170010 XMD8-92 Selleckchem Cat# S7525 Critical commercial assays Bioanalyzer high sensitivity DNA analysis Agilent Cat# 5067–4626 Brain Tumor Dissociation Kit Miltenyi Biotech Cat# 130–095–942 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9683 KAPA Library Quantification Kit for Illumina NGS Kapa Biosystems Cat# KK4835 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB Cat# E7490L NEBNext UltraTM II RNA Library Prep Kit for Illumina NEB Cat# E7775L Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q33231 Deposited data RNA sequencing of primary, GBO, and reaggregate GBOs This paper NCBI GEO Accession GEO: GSE300985 Experimental models: Cell lines Glioblastoma organoid samples This paper Table S1 Experimental models: Organisms/strains NU/J Mus musculus, female The Jackson Laboratory Cat# 002019; RRID: IMSR_JAX:002019 Software and algorithms Adobe Illustrator 28.2 64-bit Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_010279 bcl2fastq v.2.19.0.316 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html; RRID:SCR_015058 DESeq2 v.1.22.2 Love et al.41 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID:SCR_015687 (Continued on next page) Cell Reports Medicine 7, 103095, October 20, 2026 e2 Article ll OPEN ACCESS ..

Article Title: A standardized patient-derived glioblastoma organoid platform for evaluating radiotherapy efficacy and overcoming resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Doublecortin Santa Cruz Biotechnology Cat# sc-8066; RRID: AB_2088494 Goat anti-Olig2 R&D Systems Cat# AF2418; RRID: AB_2157554 Mouse anti-GFAP Sigma-Aldrich Cat# MAB360; RRID: AB_11212597 Mouse Anti-Ki-67 BD Biosciences Cat# 550609; RRID: AB_39377 Mouse anti-SOX9 Sigma-Aldrich Cat# AF146; RRID: AB_2239761 Rabbit anti-EGFRvIII Cell Signaling Cat# 64952 S; RRID: AB_2773018 Rabbit anti-HOPX Santa Cruz Biotechnology Cat# sc-30216; RRID: AB_2120833 Rat anti-Tenascin C R&D Systems Cat# MAB2138; RRID: AB_2203818 donkey anti-goat IgG Alexa Fluor 488 Thermo Fisher Cat# A-11055; RRID: AB_2336933 donkey anti-goat IgG Alexa Fluor 647 Thermo Fisher Cat# A-21447; RRID: AB_2535864 donkey anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Cat# A-31572; RRID: AB_162543 donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Cat# A-21202; RRID: AB_141607 donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher Cat# A-31570; RRID: AB_2536180 donkey anti-mouse IgG Alexa Fluor 647 Thermo Fisher Cat# A-31571; RRID: AB_162542 Chemicals, peptides, and recombinant proteins 1× RBC lysis buffer Thermo Fisher Scientific Cat# 00433357 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 ABBV-744 MedChemExpress Cat# HY-112090 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 AZD-5069 MedChemExpress Cat# HY-19855 B27 supplement without vitamin A Gibco Cat# 12587010 BGT226 MedChemExpress Cat# HY-13334 A BI-2536 MedChemExpress Cat# HY-50698 Bovine serum albumin (BSA) Sigma-Aldrich Cat# B6917; CAS# 9048–46–8 DAPI Sigma-Aldrich Cat# 10236276001; CAS# 28718–90–3 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650; CAS# 67–68–5 DMEM/F12 Medium Gibco Cat# 11330032 Donkey Serum Sigma-Aldrich Cat# S30-100ML Dulbecco’s phosphate-buffered saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat# 14190144 Formaldehyde, 16%, methanol free, Ultrapure Polysciences Cat# 18814–10; CAS#: 50–00–0 Foscarnet MedChemExpress Cat# HY-B1318 Geldanamycin MedChemExpress Cat# HY-15230 GlutaMAXTM Gibco Cat# 35050038 GSK778 MedChemExpress Cat# HY-136570 HibernateTM-A Medium Gibco Cat# A1247501 I-BET151 MedChemExpress Cat# HY-13235 Insulin solution human Sigma-Aldrich Cat# I9278 Ipatasertib MedChemExpress Cat# HY-15186 JQ-1 MedChemExpress Cat# HY-13030 KAPA SYBR FAST 2× MasterMix Thermo Fisher Scientific Cat# 50–196–5206 Masitinib MedChemExpress Cat# HY-10209 (Continued on next page) e1 Cell Reports Medicine 7, 103095, October 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Minimum Essential Medium from nonessential amino acids Gibco Cat# 11140050 N2 supplement Gibco Cat# 7502048 Neurobasal Medium Gibco Cat# 11570556 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 Paxalisib Selleckchem Cat# S8163 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 PFI-1 MedChemExpress Cat# HY-16586 RNA Clean & Concentrator 5 Zymo Research Cat# R1013 SB225002 MedChemExpress Cat# HY-16711 SMARTScribe Reverse Transcriptase Takara Cat# 639537 SPRIselect Reagent Beckman Coulter Cat# B23318 Tanespimycin MedChemExpress Cat# HY-10211 TritonTM X-100 Sigma-Aldrich Cat# T9284 Torin-2 MedChemExpress Cat# HY-13002 Tris (1 M), pH 8.0, RNase-free Thermo Fisher Scientific Cat# AM9855G TRIzol reagent Thermo Fisher Scientific Cat# 15596026 Trypan blue stain, 0.4% Thermo Fisher Scientific Cat# T10282 Tween 20 Sigma-Aldrich Cat# P9416-50mL; CAS# 9005–64–5 VECTASHIELD Vibrance Antifade Mounting Medium Vector Laboratories Cat# H170010 XMD8-92 Selleckchem Cat# S7525 Critical commercial assays Bioanalyzer high sensitivity DNA analysis Agilent Cat# 5067–4626 Brain Tumor Dissociation Kit Miltenyi Biotech Cat# 130–095–942 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9683 KAPA Library Quantification Kit for Illumina NGS Kapa Biosystems Cat# KK4835 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB Cat# E7490L NEBNext UltraTM II RNA Library Prep Kit for Illumina NEB Cat# E7775L Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q33231 Deposited data RNA sequencing of primary, GBO, and reaggregate GBOs This paper NCBI GEO Accession GEO: GSE300985 Experimental models: Cell lines Glioblastoma organoid samples This paper Table S1 Experimental models: Organisms/strains NU/J Mus musculus, female The Jackson Laboratory Cat# 002019; RRID: IMSR_JAX:002019 Software and algorithms Adobe Illustrator 28.2 64-bit Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_010279 bcl2fastq v.2.19.0.316 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html; RRID:SCR_015058 DESeq2 v.1.22.2 Love et al.41 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID:SCR_015687 (Continued on next page) Cell Reports Medicine 7, 103095, October 20, 2026 e2 Article ll OPEN ACCESS ..

Article Title: A standardized patient-derived glioblastoma organoid platform for evaluating radiotherapy efficacy and overcoming resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Doublecortin Santa Cruz Biotechnology Cat# sc-8066; RRID: AB_2088494 Goat anti-Olig2 R&D Systems Cat# AF2418; RRID: AB_2157554 Mouse anti-GFAP Sigma-Aldrich Cat# MAB360; RRID: AB_11212597 Mouse Anti-Ki-67 BD Biosciences Cat# 550609; RRID: AB_39377 Mouse anti-SOX9 Sigma-Aldrich Cat# AF146; RRID: AB_2239761 Rabbit anti-EGFRvIII Cell Signaling Cat# 64952 S; RRID: AB_2773018 Rabbit anti-HOPX Santa Cruz Biotechnology Cat# sc-30216; RRID: AB_2120833 Rat anti-Tenascin C R&D Systems Cat# MAB2138; RRID: AB_2203818 donkey anti-goat IgG Alexa Fluor 488 Thermo Fisher Cat# A-11055; RRID: AB_2336933 donkey anti-goat IgG Alexa Fluor 647 Thermo Fisher Cat# A-21447; RRID: AB_2535864 donkey anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Cat# A-31572; RRID: AB_162543 donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Cat# A-21202; RRID: AB_141607 donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher Cat# A-31570; RRID: AB_2536180 donkey anti-mouse IgG Alexa Fluor 647 Thermo Fisher Cat# A-31571; RRID: AB_162542 Chemicals, peptides, and recombinant proteins 1× RBC lysis buffer Thermo Fisher Scientific Cat# 00433357 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 ABBV-744 MedChemExpress Cat# HY-112090 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 AZD-5069 MedChemExpress Cat# HY-19855 B27 supplement without vitamin A Gibco Cat# 12587010 BGT226 MedChemExpress Cat# HY-13334 A BI-2536 MedChemExpress Cat# HY-50698 Bovine serum albumin (BSA) Sigma-Aldrich Cat# B6917; CAS# 9048–46–8 DAPI Sigma-Aldrich Cat# 10236276001; CAS# 28718–90–3 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650; CAS# 67–68–5 DMEM/F12 Medium Gibco Cat# 11330032 Donkey Serum Sigma-Aldrich Cat# S30-100ML Dulbecco’s phosphate-buffered saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat# 14190144 Formaldehyde, 16%, methanol free, Ultrapure Polysciences Cat# 18814–10; CAS#: 50–00–0 Foscarnet MedChemExpress Cat# HY-B1318 Geldanamycin MedChemExpress Cat# HY-15230 GlutaMAXTM Gibco Cat# 35050038 GSK778 MedChemExpress Cat# HY-136570 HibernateTM-A Medium Gibco Cat# A1247501 I-BET151 MedChemExpress Cat# HY-13235 Insulin solution human Sigma-Aldrich Cat# I9278 Ipatasertib MedChemExpress Cat# HY-15186 JQ-1 MedChemExpress Cat# HY-13030 KAPA SYBR FAST 2× MasterMix Thermo Fisher Scientific Cat# 50–196–5206 Masitinib MedChemExpress Cat# HY-10209 (Continued on next page) e1 Cell Reports Medicine 7, 103095, October 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Minimum Essential Medium from nonessential amino acids Gibco Cat# 11140050 N2 supplement Gibco Cat# 7502048 Neurobasal Medium Gibco Cat# 11570556 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 Paxalisib Selleckchem Cat# S8163 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 PFI-1 MedChemExpress Cat# HY-16586 RNA Clean & Concentrator 5 Zymo Research Cat# R1013 SB225002 MedChemExpress Cat# HY-16711 SMARTScribe Reverse Transcriptase Takara Cat# 639537 SPRIselect Reagent Beckman Coulter Cat# B23318 Tanespimycin MedChemExpress Cat# HY-10211 TritonTM X-100 Sigma-Aldrich Cat# T9284 Torin-2 MedChemExpress Cat# HY-13002 Tris (1 M), pH 8.0, RNase-free Thermo Fisher Scientific Cat# AM9855G TRIzol reagent Thermo Fisher Scientific Cat# 15596026 Trypan blue stain, 0.4% Thermo Fisher Scientific Cat# T10282 Tween 20 Sigma-Aldrich Cat# P9416-50mL; CAS# 9005–64–5 VECTASHIELD Vibrance Antifade Mounting Medium Vector Laboratories Cat# H170010 XMD8-92 Selleckchem Cat# S7525 Critical commercial assays Bioanalyzer high sensitivity DNA analysis Agilent Cat# 5067–4626 Brain Tumor Dissociation Kit Miltenyi Biotech Cat# 130–095–942 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9683 KAPA Library Quantification Kit for Illumina NGS Kapa Biosystems Cat# KK4835 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB Cat# E7490L NEBNext UltraTM II RNA Library Prep Kit for Illumina NEB Cat# E7775L Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q33231 Deposited data RNA sequencing of primary, GBO, and reaggregate GBOs This paper NCBI GEO Accession GEO: GSE300985 Experimental models: Cell lines Glioblastoma organoid samples This paper Table S1 Experimental models: Organisms/strains NU/J Mus musculus, female The Jackson Laboratory Cat# 002019; RRID: IMSR_JAX:002019 Software and algorithms Adobe Illustrator 28.2 64-bit Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_010279 bcl2fastq v.2.19.0.316 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html; RRID:SCR_015058 DESeq2 v.1.22.2 Love et al.41 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID:SCR_015687 (Continued on next page) Cell Reports Medicine 7, 103095, October 20, 2026 e2 Article ll OPEN ACCESS ..

Article Title: A standardized patient-derived glioblastoma organoid platform for evaluating radiotherapy efficacy and overcoming resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Doublecortin Santa Cruz Biotechnology Cat# sc-8066; RRID: AB_2088494 Goat anti-Olig2 R&D Systems Cat# AF2418; RRID: AB_2157554 Mouse anti-GFAP Sigma-Aldrich Cat# MAB360; RRID: AB_11212597 Mouse Anti-Ki-67 BD Biosciences Cat# 550609; RRID: AB_39377 Mouse anti-SOX9 Sigma-Aldrich Cat# AF146; RRID: AB_2239761 Rabbit anti-EGFRvIII Cell Signaling Cat# 64952 S; RRID: AB_2773018 Rabbit anti-HOPX Santa Cruz Biotechnology Cat# sc-30216; RRID: AB_2120833 Rat anti-Tenascin C R&D Systems Cat# MAB2138; RRID: AB_2203818 donkey anti-goat IgG Alexa Fluor 488 Thermo Fisher Cat# A-11055; RRID: AB_2336933 donkey anti-goat IgG Alexa Fluor 647 Thermo Fisher Cat# A-21447; RRID: AB_2535864 donkey anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Cat# A-31572; RRID: AB_162543 donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Cat# A-21202; RRID: AB_141607 donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher Cat# A-31570; RRID: AB_2536180 donkey anti-mouse IgG Alexa Fluor 647 Thermo Fisher Cat# A-31571; RRID: AB_162542 Chemicals, peptides, and recombinant proteins 1× RBC lysis buffer Thermo Fisher Scientific Cat# 00433357 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 ABBV-744 MedChemExpress Cat# HY-112090 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 AZD-5069 MedChemExpress Cat# HY-19855 B27 supplement without vitamin A Gibco Cat# 12587010 BGT226 MedChemExpress Cat# HY-13334 A BI-2536 MedChemExpress Cat# HY-50698 Bovine serum albumin (BSA) Sigma-Aldrich Cat# B6917; CAS# 9048–46–8 DAPI Sigma-Aldrich Cat# 10236276001; CAS# 28718–90–3 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650; CAS# 67–68–5 DMEM/F12 Medium Gibco Cat# 11330032 Donkey Serum Sigma-Aldrich Cat# S30-100ML Dulbecco’s phosphate-buffered saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat# 14190144 Formaldehyde, 16%, methanol free, Ultrapure Polysciences Cat# 18814–10; CAS#: 50–00–0 Foscarnet MedChemExpress Cat# HY-B1318 Geldanamycin MedChemExpress Cat# HY-15230 GlutaMAXTM Gibco Cat# 35050038 GSK778 MedChemExpress Cat# HY-136570 HibernateTM-A Medium Gibco Cat# A1247501 I-BET151 MedChemExpress Cat# HY-13235 Insulin solution human Sigma-Aldrich Cat# I9278 Ipatasertib MedChemExpress Cat# HY-15186 JQ-1 MedChemExpress Cat# HY-13030 KAPA SYBR FAST 2× MasterMix Thermo Fisher Scientific Cat# 50–196–5206 Masitinib MedChemExpress Cat# HY-10209 (Continued on next page) e1 Cell Reports Medicine 7, 103095, October 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Minimum Essential Medium from nonessential amino acids Gibco Cat# 11140050 N2 supplement Gibco Cat# 7502048 Neurobasal Medium Gibco Cat# 11570556 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 Paxalisib Selleckchem Cat# S8163 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 PFI-1 MedChemExpress Cat# HY-16586 RNA Clean & Concentrator 5 Zymo Research Cat# R1013 SB225002 MedChemExpress Cat# HY-16711 SMARTScribe Reverse Transcriptase Takara Cat# 639537 SPRIselect Reagent Beckman Coulter Cat# B23318 Tanespimycin MedChemExpress Cat# HY-10211 TritonTM X-100 Sigma-Aldrich Cat# T9284 Torin-2 MedChemExpress Cat# HY-13002 Tris (1 M), pH 8.0, RNase-free Thermo Fisher Scientific Cat# AM9855G TRIzol reagent Thermo Fisher Scientific Cat# 15596026 Trypan blue stain, 0.4% Thermo Fisher Scientific Cat# T10282 Tween 20 Sigma-Aldrich Cat# P9416-50mL; CAS# 9005–64–5 VECTASHIELD Vibrance Antifade Mounting Medium Vector Laboratories Cat# H170010 XMD8-92 Selleckchem Cat# S7525 Critical commercial assays Bioanalyzer high sensitivity DNA analysis Agilent Cat# 5067–4626 Brain Tumor Dissociation Kit Miltenyi Biotech Cat# 130–095–942 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9683 KAPA Library Quantification Kit for Illumina NGS Kapa Biosystems Cat# KK4835 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB Cat# E7490L NEBNext UltraTM II RNA Library Prep Kit for Illumina NEB Cat# E7775L Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q33231 Deposited data RNA sequencing of primary, GBO, and reaggregate GBOs This paper NCBI GEO Accession GEO: GSE300985 Experimental models: Cell lines Glioblastoma organoid samples This paper Table S1 Experimental models: Organisms/strains NU/J Mus musculus, female The Jackson Laboratory Cat# 002019; RRID: IMSR_JAX:002019 Software and algorithms Adobe Illustrator 28.2 64-bit Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_010279 bcl2fastq v.2.19.0.316 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html; RRID:SCR_015058 DESeq2 v.1.22.2 Love et al.41 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID:SCR_015687 (Continued on next page) Cell Reports Medicine 7, 103095, October 20, 2026 e2 Article ll OPEN ACCESS ..

Article Title: A standardized patient-derived glioblastoma organoid platform for evaluating radiotherapy efficacy and overcoming resistance.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-Doublecortin Santa Cruz Biotechnology Cat# sc-8066; RRID: AB_2088494 Goat anti-Olig2 R&D Systems Cat# AF2418; RRID: AB_2157554 Mouse anti-GFAP Sigma-Aldrich Cat# MAB360; RRID: AB_11212597 Mouse Anti-Ki-67 BD Biosciences Cat# 550609; RRID: AB_39377 Mouse anti-SOX9 Sigma-Aldrich Cat# AF146; RRID: AB_2239761 Rabbit anti-EGFRvIII Cell Signaling Cat# 64952 S; RRID: AB_2773018 Rabbit anti-HOPX Santa Cruz Biotechnology Cat# sc-30216; RRID: AB_2120833 Rat anti-Tenascin C R&D Systems Cat# MAB2138; RRID: AB_2203818 donkey anti-goat IgG Alexa Fluor 488 Thermo Fisher Cat# A-11055; RRID: AB_2336933 donkey anti-goat IgG Alexa Fluor 647 Thermo Fisher Cat# A-21447; RRID: AB_2535864 donkey anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Cat# A-31572; RRID: AB_162543 donkey anti-mouse IgG Alexa Fluor 488 Thermo Fisher Cat# A-21202; RRID: AB_141607 donkey anti-mouse IgG Alexa Fluor 555 Thermo Fisher Cat# A-31570; RRID: AB_2536180 donkey anti-mouse IgG Alexa Fluor 647 Thermo Fisher Cat# A-31571; RRID: AB_162542 Chemicals, peptides, and recombinant proteins 1× RBC lysis buffer Thermo Fisher Scientific Cat# 00433357 2-Mercaptoethanol Thermo Fisher Scientific Cat# 21985023 ABBV-744 MedChemExpress Cat# HY-112090 Antibiotic-Antimycotic (100×) Gibco Cat# 15240062 AZD-5069 MedChemExpress Cat# HY-19855 B27 supplement without vitamin A Gibco Cat# 12587010 BGT226 MedChemExpress Cat# HY-13334 A BI-2536 MedChemExpress Cat# HY-50698 Bovine serum albumin (BSA) Sigma-Aldrich Cat# B6917; CAS# 9048–46–8 DAPI Sigma-Aldrich Cat# 10236276001; CAS# 28718–90–3 Dimethyl sulfoxide (DMSO) Sigma-Aldrich Cat# D2650; CAS# 67–68–5 DMEM/F12 Medium Gibco Cat# 11330032 Donkey Serum Sigma-Aldrich Cat# S30-100ML Dulbecco’s phosphate-buffered saline (DPBS), no calcium, no magnesium Thermo Fisher Scientific Cat# 14190144 Formaldehyde, 16%, methanol free, Ultrapure Polysciences Cat# 18814–10; CAS#: 50–00–0 Foscarnet MedChemExpress Cat# HY-B1318 Geldanamycin MedChemExpress Cat# HY-15230 GlutaMAXTM Gibco Cat# 35050038 GSK778 MedChemExpress Cat# HY-136570 HibernateTM-A Medium Gibco Cat# A1247501 I-BET151 MedChemExpress Cat# HY-13235 Insulin solution human Sigma-Aldrich Cat# I9278 Ipatasertib MedChemExpress Cat# HY-15186 JQ-1 MedChemExpress Cat# HY-13030 KAPA SYBR FAST 2× MasterMix Thermo Fisher Scientific Cat# 50–196–5206 Masitinib MedChemExpress Cat# HY-10209 (Continued on next page) e1 Cell Reports Medicine 7, 103095, October 20, 2026 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Minimum Essential Medium from nonessential amino acids Gibco Cat# 11140050 N2 supplement Gibco Cat# 7502048 Neurobasal Medium Gibco Cat# 11570556 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 Paxalisib Selleckchem Cat# S8163 Penicillin-Streptomycin (5,000 U/mL) Thermo Fisher Scientific Cat# 15070063 PFI-1 MedChemExpress Cat# HY-16586 RNA Clean & Concentrator 5 Zymo Research Cat# R1013 SB225002 MedChemExpress Cat# HY-16711 SMARTScribe Reverse Transcriptase Takara Cat# 639537 SPRIselect Reagent Beckman Coulter Cat# B23318 Tanespimycin MedChemExpress Cat# HY-10211 TritonTM X-100 Sigma-Aldrich Cat# T9284 Torin-2 MedChemExpress Cat# HY-13002 Tris (1 M), pH 8.0, RNase-free Thermo Fisher Scientific Cat# AM9855G TRIzol reagent Thermo Fisher Scientific Cat# 15596026 Trypan blue stain, 0.4% Thermo Fisher Scientific Cat# T10282 Tween 20 Sigma-Aldrich Cat# P9416-50mL; CAS# 9005–64–5 VECTASHIELD Vibrance Antifade Mounting Medium Vector Laboratories Cat# H170010 XMD8-92 Selleckchem Cat# S7525 Critical commercial assays Bioanalyzer high sensitivity DNA analysis Agilent Cat# 5067–4626 Brain Tumor Dissociation Kit Miltenyi Biotech Cat# 130–095–942 CellTiter-Glo 3D Cell Viability Assay Promega Cat# G9683 KAPA Library Quantification Kit for Illumina NGS Kapa Biosystems Cat# KK4835 NEBNext Poly(A) mRNA Magnetic Isolation Module NEB Cat# E7490L NEBNext UltraTM II RNA Library Prep Kit for Illumina NEB Cat# E7775L Qubit dsDNA HS Assay Kit Thermo Fisher Scientific Cat# Q33231 Deposited data RNA sequencing of primary, GBO, and reaggregate GBOs This paper NCBI GEO Accession GEO: GSE300985 Experimental models: Cell lines Glioblastoma organoid samples This paper Table S1 Experimental models: Organisms/strains NU/J Mus musculus, female The Jackson Laboratory Cat# 002019; RRID: IMSR_JAX:002019 Software and algorithms Adobe Illustrator 28.2 64-bit Adobe https://www.adobe.com/products/ illustrator.html; RRID: SCR_010279 bcl2fastq v.2.19.0.316 Illumina https://support.illumina.com/sequencing/ sequencing_software/bcl2fastqconversion-software.html; RRID:SCR_015058 DESeq2 v.1.22.2 Love et al.41 https://bioconductor.org/packages/ release/bioc/html/DESeq2.html; RRID:SCR_015687 (Continued on next page) Cell Reports Medicine 7, 103095, October 20, 2026 e2 Article ll OPEN ACCESS ..