smartscribe reverse transcriptase (TaKaRa)
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TaKaRa
smartscribe reverse transcriptase
Smartscribe Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smartscribe+reverse+transcriptase/SMARTScribe+Reverse+Transcriptase/pmc13076092-32-22-25
Average 96 stars, based on 1126 article reviews
Smartscribe Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smartscribe+reverse+transcriptase/SMARTScribe+Reverse+Transcriptase/pmc13076092-32-22-25
Average 96 stars, based on 1126 article reviews
smartscribe reverse transcriptase - by Bioz Stars,
2026-09
96/100 stars
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Immunoprecipitation:Article Title: METTL3 mediates m 6 A methylation of ADAM17 to Downregulate ACE2–Ang-(1–7) axis and promote progression of hypertension Article Snippet: A minimum of 1 μg total RNA was fragmented with an NEBNext Magnesium RNA fragmentation module (New England Biolabs, Ipswich, MA, USA) by incubating at 94 °C for 5 min. Fragmented RNA, including rRNA, was enriched with m6A antibody–Dynabead complexes through immunoprecipitation. .. Immunoprecipitated RNA was then reverse-transcribed to yield first-strand cDNA via Reverse Transcription:Article Title: METTL3 mediates m 6 A methylation of ADAM17 to Downregulate ACE2–Ang-(1–7) axis and promote progression of hypertension Article Snippet: A minimum of 1 μg total RNA was fragmented with an NEBNext Magnesium RNA fragmentation module (New England Biolabs, Ipswich, MA, USA) by incubating at 94 °C for 5 min. Fragmented RNA, including rRNA, was enriched with m6A antibody–Dynabead complexes through immunoprecipitation. .. Immunoprecipitated RNA was then reverse-transcribed to yield first-strand cDNA via Article Title: Pan-cancer macrophage atlas uncovers that MHC-II high TAMs induce Treg activation through physical interactions. Article Snippet: For bulk RNA-seq, RNA extraction was performed using FastPure Cell/Tissue Total RNA Isolation Kit V2 (Vazyme RC112-01). .. Reverse transcription was performed using Article Title: Mesenchymal stroma cell-derived stem cell factor mediates cross-species compatibility of the hematopoietic stem cell niche Article Snippet: RNA was prepared using the miRNeasy Micro Kit (Qiagen). .. Total RNA was eluted in 10ul and 5ul were used for library preparation for next generation sequencing. cDNA was synthesized from 5 μl total RNA using the Article Title: A Recombinant Antibody Against Human DRP1 Serine 616 Phosphorylation Enables Detection of BRAF V600E -Associated Mitochondrial Division in Cancer Article Snippet: Sequencing of the 3G11 antibody was performed using SMARTer 5′ RACE technology (Takara Bio, MoutainView, CA, USA, Cat. No. 634858) adapted for immunoglobulins to amplify the variable genes from the heavy and kappa chains from the 3G11 hybridoma. .. Briefly, RNA was extracted from the hybridoma using a RNeasy Mini Kit (Qiagen, Hilden, Germany, Cat. No. 74104), followed by first-strand cDNA synthesis using constant gene-specific 3′ primers (GSP1) based on mouse IgG/mouse kappa constant isotypes and incubation with the SMARTer II A Oligonucleotide and Article Title: Direct-to-library methods, systems, and compositions Article Snippet: 2.0 μL 1 μM Poly dT primer (IDT) and 6 μL of SMARTScribe 1st Strand buffer (5×) (Takara, Kusatsu, Japan) was added to the eluate and the resulting mixture incubated at 95° C. for 1 minute before placing on ice. .. The Extension and Template-switching mix was prepared by combining 4.5 μL SMARTScribe 1st Strand buffer (5×) (Takara, Kusatsu, Japan), 0.5 μL dNTP mix (25 mM per nucleotide, Thermo-Fisher Scientific, Waltham, MA), 2.0 μL Article Title: Direct-to-library methods, systems, and compositions Article Snippet: .. For extension and template switching, 0.5×SMARTScribe RT buffer (Takara, Japan), 2 μM DTT (Takara, Japan), 0.25 mM dNTPs (Thermo-Fisher, Waltham, MA), 200 units of Article Title: The circadian isoform landscape of mouse livers Article Snippet: .. First strand reverse transcription was performed for 1 h at 42°C, before being heat inactivated for 5 min at 70°C, using the Article Title: Human antibody targeting Crimean-Congo hemorrhagic fever virus glycoprotein 38 protects mice against heterologous virus challenge Article Snippet: .. Post incubation, added to the total RNA reaction was a mixture of 5× first-strand buffer (Clontech), dithiothreitol (20 mM), 5′ template switch oligo (10 μM), deoxynucleotide triphosphate (dNTP) solution (10 mM each), and 10× Next-Generation Sequencing:Article Title: Mesenchymal stroma cell-derived stem cell factor mediates cross-species compatibility of the hematopoietic stem cell niche Article Snippet: RNA was prepared using the miRNeasy Micro Kit (Qiagen). .. Total RNA was eluted in 10ul and 5ul were used for library preparation for next generation sequencing. cDNA was synthesized from 5 μl total RNA using the Synthesized:Article Title: Mesenchymal stroma cell-derived stem cell factor mediates cross-species compatibility of the hematopoietic stem cell niche Article Snippet: RNA was prepared using the miRNeasy Micro Kit (Qiagen). .. Total RNA was eluted in 10ul and 5ul were used for library preparation for next generation sequencing. cDNA was synthesized from 5 μl total RNA using the Amplification:Article Title: Mesenchymal stroma cell-derived stem cell factor mediates cross-species compatibility of the hematopoietic stem cell niche Article Snippet: RNA was prepared using the miRNeasy Micro Kit (Qiagen). .. Total RNA was eluted in 10ul and 5ul were used for library preparation for next generation sequencing. cDNA was synthesized from 5 μl total RNA using the cDNA Synthesis:Article Title: A Recombinant Antibody Against Human DRP1 Serine 616 Phosphorylation Enables Detection of BRAF V600E -Associated Mitochondrial Division in Cancer Article Snippet: Sequencing of the 3G11 antibody was performed using SMARTer 5′ RACE technology (Takara Bio, MoutainView, CA, USA, Cat. No. 634858) adapted for immunoglobulins to amplify the variable genes from the heavy and kappa chains from the 3G11 hybridoma. .. Briefly, RNA was extracted from the hybridoma using a RNeasy Mini Kit (Qiagen, Hilden, Germany, Cat. No. 74104), followed by first-strand cDNA synthesis using constant gene-specific 3′ primers (GSP1) based on mouse IgG/mouse kappa constant isotypes and incubation with the SMARTer II A Oligonucleotide and Incubation:Article Title: A Recombinant Antibody Against Human DRP1 Serine 616 Phosphorylation Enables Detection of BRAF V600E -Associated Mitochondrial Division in Cancer Article Snippet: Sequencing of the 3G11 antibody was performed using SMARTer 5′ RACE technology (Takara Bio, MoutainView, CA, USA, Cat. No. 634858) adapted for immunoglobulins to amplify the variable genes from the heavy and kappa chains from the 3G11 hybridoma. .. Briefly, RNA was extracted from the hybridoma using a RNeasy Mini Kit (Qiagen, Hilden, Germany, Cat. No. 74104), followed by first-strand cDNA synthesis using constant gene-specific 3′ primers (GSP1) based on mouse IgG/mouse kappa constant isotypes and incubation with the SMARTer II A Oligonucleotide and Article Title: Direct-to-library methods, systems, and compositions Article Snippet: .. For extension and template switching, 0.5×SMARTScribe RT buffer (Takara, Japan), 2 μM DTT (Takara, Japan), 0.25 mM dNTPs (Thermo-Fisher, Waltham, MA), 200 units of Article Title: Human antibody targeting Crimean-Congo hemorrhagic fever virus glycoprotein 38 protects mice against heterologous virus challenge Article Snippet: .. Post incubation, added to the total RNA reaction was a mixture of 5× first-strand buffer (Clontech), dithiothreitol (20 mM), 5′ template switch oligo (10 μM), deoxynucleotide triphosphate (dNTP) solution (10 mM each), and 10× |